Index
Project information
Purpose
Apparatus
Method
Hypothesis
Observation
Conclusion
References
1. Using the permenent marker, divide the cutting board into six sections,
numbering each section #1-6.
2. Put on glives and wipe the piece of lunch meat all over the surface of the cutting board. Wipe evenly over the entire surface in circular motions. Leave out overnight.
3. The next day cleanse each section of the cuttingboard with a different disinfectant and then culture the bacteria from each section on a nutrient agar petri dish.
4. Preparethe disinfectant soultions by numbering six small cups #1-6 using permanent marker. Each numbered cup will match one section of your cutting board formite.
5. Fill ewach cup with a different disinfectant soultion and write it in a data table. Fill the first cup with water as a negative control.
6. Using the tweezers, grab a cotton ball from a NEW, unopened bag of cotton balls. Dip it into one of the solutions, and rub it on the surface of the cutting board in the matching numbered section. Be careful not to let the solution run into another section.
7. After each application, throw the cotton ball into the trassh and dip the twezers into an extra cup filled with full strength rubbing alchol.
8. Repeat steps 6 & 7 until you have applied a different disinfectant to each square of the cutting board.
9. When all sections of the cutting board are dry, you are ready to culture baform each disinfectant treatment. Prepare the agar plates by numbering the lids #1 -6 to match the sections of your cutting board. Arrange the plates on a cookie sheet lined with clean paper towels. DO NOT open the lids yet or the cultures will be contaminated.
10. Using the tweezers, grab a clean Q-tip from a NEW, unopened box of Q-tips. Holding one end only, swipe the other end across one section of the cutting board using a circular motion. DO NOT allow the tip of the swab to contact anything else!
11. With a free hand, open the lid of the matching agar petri dish and swipe the Q-tip across the agar surface using a zig-zag motion. Immediatly replace the lid of the petri dish and secure with a few peices of clear tape. DO NOT set the lid down while streaking the agar because this can contaminate the lid and change the results!
12. Repeat steps 10 and 11 until you have swiped each sectionof the cutting boardonto a seperate agar plate.
13. leave the plates on the cookie sheet in a warm place for 1-2 days, until bacterial colonies are visible.
14. Count the number of colonies on each plate and write the results in the data table, along with any other observations.