First, the tissue culture flasks were filled with 80 ml of 10 % BBM solution.
The flasks were labeled accordingly. The first pair of flasks was labeled “Control 1 and 2”. The second pair of flasks was labeled “Sun light detergent 1 and 2”. The third pair of flasks was labeled “No Name 1 and 2”. The last pair of flasks was labeled “Tide detergent 1 and 2”.
An Analytical balance was used to weigh 1.38 grams of each detergent. A spatula was used to scoop the detergent. Each was then placed in a small jar. This step was done twice to have a back up measurement of each detergent, in case something wrong happens while doing the experiment.
After measuring, each of the detergent was added in 100 ml of hot mineral water (to dissolved detergent faster) and mixed with a stir stick.
The flasks, without tops, and the detergent solution were left sitting, at room temperature, overnight.
Then 2 ml of Chlorella Kessleri Algae was added in each flask using a medicine dropper.
The two flasks with the name “Control” are not going to be receiving any detergent, so they were put aside. In the next pair of flasks labeled “Sunlight detergent 1 and 2”, 5 drops of Sunlight detergent solution were added using a dropper. In the next pair labeled “Tide detergent 1 and 2”, 5 drops of Tide detergent solution were added using a dropper. Then in the last pair of flasks labeled, “No Name detergent 1 and 2”, 5 drops of the No Name detergent solution were added. * Note a new clean dropper was used every time a new kind of detergent was added to each corresponding flasks.*
At night, the flasks were left under a fluorescent bulb and moved to a sunlit glass door in the morning for the algae to grow. The algae were left to grow for four days before measuring the dissolve oxygen of each flask.
The oxygen level was measured in each flask, after 4 days, using a dissolved oxygen meter. Data about how each algae flask looked everyday was recorded in a notebook and pictures were taken as well.