Mean Concentration of Dye Found in Rinse for Fluorescein Washes
Dye
|
Description |
Mean (%) |
Fluorescein |
Water |
55.1 |
Fluorescein |
Soap and Water |
81.4 |
Fluorescein |
50/50 Vinegar/Water |
0.0 |
Fluorescein |
Fruit Wash |
49.3 |
Fluorescein |
Petri |
91.2 |
Concentration of Dye found in Rinse for Fluorescein Trials
Dye
|
Description |
Trial 1 |
Trial 2 |
Trial 3 |
Trial 4 |
Fluorescein |
Water |
92.56 |
59.60 |
77.38 |
69.7 |
Fluorescein |
Soap and Water |
114.58 |
101.48 |
105.51 |
113.98 |
Fluorescein |
50/50 Vinegar/Water |
n/a |
n/a |
n/a |
n/a |
Fluorescein |
Fruit Wash |
60.09 |
45.54 |
80.28 |
83.66 |
Fluorescein |
Petri |
120.99 |
122.85 |
113.62 |
113.75 |
***When reading the data, the ideal amount of dye found in rinse would be 129.23.
Looking at these results tells us that for the Fluorescein dye, a mixture of soap and water, best removed the dye. The distilled water was also a fair alternative, although somewhat unreliable, as shown above. The fruit had a similar result, only slightly lower though it appears more reliable. The vinegar did not work at all for Fluorescein but this was because of the way it reacted with the dye, skewing the readings on the Spectrofluorophotometer. The Petri dish simply shows how much dye you can get off a flat, non-porous surface.
Mean Concentration of Dye Found in Rinse for Rhodamine Washes
Dye
|
Description |
Mean (%) |
Rhodamine |
Water |
97.6 |
Rhodamine |
Soap and Water |
86.3 |
Rhodamine |
50/50 Vinegar/Water |
45.5 |
Rhodamine |
Fruit Wash |
93.4 |
Rhodamine |
Petri |
99.2 |
Concentration of Dye found in Rinse for Rhodamine Trials
Dye
|
Description |
Trial 1 |
Trial 2 |
Trial 3 |
Trial 4 |
Rhodamine |
Water |
1293.6 |
1397.9 |
1327.7 |
1283.1 |
Rhodamine |
Soap and Water |
1280.7 |
1214.5 |
1089.1 |
1105.2 |
Rhodamine |
50/50 Vinegar/Water |
622.5 |
823.37 |
188.64 |
835.94 |
Rhodamine |
Fruit Wash |
1301.5 |
1243.9 |
1270.9 |
1255.7 |
Rhodamine |
Petri |
1264.8 |
1359.8 |
1367.4 |
1394.9 |
*** When reading the data, the ideal amount of dye found in rinse would be 1358.
After interpreting the data, we found that the distilled water wash worked the best because Rhodamine is water-soluble dye. The fruit wash had a much better end result then it did for Fluorescein and appears not to be suitable for all types of pesticides. The soap and water wash had a similar result as it did in the Fluorescein trials, which shows it would be a good all-around wash. The vinegar wash showed stable results for Rhodamine but still proved to be unreliable and a bad choice for a wash. |