1) Use an electronic pipette to apply 16 two micro litre drops of Rhodamine dye on four sets of five apples. Place the drops in a four by four formation and let dry for 24 hours.

2) After each set of five apples, place 16 two micro liter drops in a vial. Immediately, dilute the vial three times and pour into a volumetric flask and then fill the flask to 100 milliliters. As well, after every five apples, place 16, 2 micro litre, drops onto a large petri dish. Let dry for 24 hours.

3) After completing this, begin making a standard curve by taking the Rhodamine dye and diluting exponentially in the different treatments. Start in a concentration of zero, 100, 500, 1000, and 1500 micro liters.

4) Repeat all the steps again using the Fluorescein except when making the standard curve use concentration of zero, 12.50, 62.50, 125, and 187.5.

5) After 24 hours, prepare the four treatments of 50/ 50 distilled water and vinegar, .05 mL of organic fruit wash to every 100 mL of distilled water, .05 mL of dish detergent to every 100 mL of distilled water, and plain distilled water. The control is untreated apples in water and does not need to be prepared.

6) Set out five containers and fill with 100 mL of treatment.

7) Inside each container place one apple with Rhodamine dye. Be careful not to touch the dye.

8) Place lids on containers and shake apples for one minute.

9) Remove apples and using a manual pipette squirt 20 mL of rinse from each container into small vials. Label vials appropriately.

10) Repeat steps six to nine with each treatment. After each treatment wash all equipment used in washing the apples.

11) Take the volumetric flasks with the standard curve and take 20 mL samples and place in small vials.

12) Take all the vials from washing the apples and the standard curve and place in a rack for the Spectrofluorophotometer, which is used to show the concentration and the fluorescent intensity of dye in the sample.

13) Place the rack on the Spectrofluorophotometer and begin running the machine for results.  After results are computed, print and label them.

14) Repeat steps six to thirteen with the Fluorescein apples and standard curve.

15) Graph and analyze results.