For this experiment, the results showed that soap and water worked best to rinse off the Fluorescein dye and water worked best to take off the Rhodamine dye. Overall, the soap and water would be the best alternative for washing fruit, especially because of its prevalence in households. Our hypothesis was that if different fluorescent dyes are applied to apples and rinsed of with various washes, then the commercial wash will remove the most dye. This proved to be incorrect for both trials as the commercial fruit wash was inconsistent and had poor results. This information can be applied to washing pesticides off fruit in common households because the dye simulated pesticides. Like there are different kinds of pesticides, we also used different kinds of dye to find the best overall wash. Looking back, choosing Rhodamine appears to have been a mistake because it is water-soluble, which produced obvious but not necessarily applicable results. Using vinegar was also a mistake because we suspected from the beginning that the readings on the Spectrofluorophotometer would be skewed. We thought the results would be skewed because of vinigar's fluorescent quality while in actuality it was the pH level of the vinegar. To correct this we could have used a buffer. For a next experiment, we would try more dyes that are oil-based, add some more washes and use a buffer. The more tests we could do, the more accurately we could be certain what wash worked the best.
|