Methods
1.
Collect tree bark from Prince Albert
2.
Dry and grind tree bark
3.
Extract antioxidants from ground tree bark using water
4.
Use the Folin-Ciocalteau Assay to determine the total amount of
polyphenols
5. Use the Ferric Reducing Ability of Plasma (FRAP) Assay to determine the antioxidating activity (Reference 1).
6. Do bacterial susceptibility tests on four clinically important bacteria strains (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Enterococcus faecalis) (Reference 2).
7.
Use the Ames Test to see if the extracts are antimutagenic (using Salmonella
typhimurium TA 1535)
