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Peeling Away Problems The Antioxidating, Antimicrobial, and Antimutagenic Effects of Tree Bark |
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Bacterial Susceptibility Tests (See Reference 2 for details) 1.
Spread 200µL of
overnight grown bacterial culture evenly on LB Agar plates 2.
Wells (6mm
diameter) are made in the agar 3.
50µL of the
extracts are pipetted into wells 4.
Plates are
incubated overnight at 37ºC LB Agar
Plate
1 L
water (H2O)
10
g
tryptone
5
g
yeast extract
10
g
NaCl
mix add 15 g of Agar, autoclave for 30 minutes, pour into 100x15 mm petri dish |