It
is the
tension 
between creativity and skepticism that has produced the stunning
and unexpected
findings of science. —Carl Sagan
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Due to contamination reducing the
number of trials that could be used for trend setting, the reliability of the
lab results were slightly reduced. As can be seen from the results of Jan 17th/07
for 60min sonication, the bottom two wells of the first column on the 96-well
plate gave extraordinarily high OD readings. This may mean that while the E.
Coli was inhibited by the ultrasound or spectinomycin, a foreign bacteria
species with a higher MIC than E. Coli was
induced into and reproduced in the solution. Or, more likely, the higher OD
readings can be explained by the possibility that some of the E.
coli that was intended for the second column had jumped into these two
wells. The described is a minor source of error (SOE), because contaminated
wells are relatively easy to identify and eliminate from account. Contamination
occurs most easily when the pipette tips come into contact with a material that
was not planned for it to come into contact with, such as human skin and cloth.
With this known, this SOE is easily reduced.
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Enhancement of Antibiotic Action with an Application of
Ultrasound 29/04/2007 |
