<--TITLE-->

v a r i a b l e s

<--menu--> <--mainarea-->



CONTROLLED VARIABLES
  • Reagent concentrations and volumes (all except the magnesium chloride and water)
  • PCR Cycling conditions (temperatures, incubation period, cycle numbers)
  • Equipment use
  • Reaction Volumes
  • Dilution series
  • No Template Control



MANIPULATED VARIABLES
  • Magnesium chloride concentration
  • Water volumes



RESPONDING VARIABLES
  • Fluorescence / Amount of PCR product
  • Efficiency