h y p o t h e s i s

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Since the project was more of a retrospective look at PCR and laboratories that carry out PCR would have optimized reagent concentrations/volumes therefore I will not be generating a prediction as to the precise optimal magnesium concentration.

Generally though, I predict that as the magnesium chloride concentration increases, the efficiency will improve or be more optimal simply because some of the reagents of PCR require free magnesium for function.

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