In this project the Winter wheat, Norstar, and the Spring wheat, Manitou, were used to study the expression of two specific cold-tolerant genes (Wcs120 and Wcor410). The objective was to understand expression patterns of low temperature-induced genes in winter and spring wheat exposed to 4 degrees Celcius. The hypothesis was that the Norstar will express specific cold-regulated genes at a higher level than the Manitou.

     Seeds from the two wheat varieties were germinated for 15 days and exposed to 4 degrees Celcius for 0, 15, 30, 45 and 60 minute trials. Leaves were harvested and RNA was extracted. From the extracted Ribonucleic acid (RNA), copy-Deoxyribonucleic acid (cDNA) was synthesized to use in Real-Time Polymerase Chain Reaction (PCR) to measure expression of cold-induced genes Wcs120 and Wcor410. The Real-Time PCR data was then used to calculate the relative expression of the genes.

     The results show that in all the treatments the Norstar winter wheat had a higher expression over the Manitou spring wheat. Both low temperature-induced genes, Wcs120 and Wcor410, showed that there were significant differences in the expression between the two types. This difference allows farmers to have an earlier, therefore more productive, yield the next year from the winter wheat.

.:Created by Mithum Kularatne - 2006 Virtual Science Fair:.