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The type of detergent used during the DNA extraction
process from a sample of kiwi cells is just one among many factors that
influence the amount we can obtain. The type of fruit or vegetable used, the
temperature of the water baths, the kind of alcohol added, as well as the mashing
process prior to the extraction, all affect how much DNA will be liberated
and then precipitated.
Out of plain curiosity, I repeated the same experiment,
however, instead of mashed kiwi I used onion that I chopped in a
blender. Three out of three trials were unsuccessful - although I used
identical detergents, I was not able to precipitate a lot of DNA strands.
Furthermore, the strands were short and slippery, and I couldn't take them
out of the test tube.
There are two possible explanations - either onion is not a suitable vegetable to perform the experiment or the blades of the blender
simply tore apart the DNA strands and they became too small to be taken out.
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Should we blend fruit or vegetables prior
the DNA extraction?
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By analyzing all the factors mentioned above, we
could develop a perfect procedure to perform a plentiful DNA extraction
- both easy and cheap. It could certainly help to reduce the cost of
buying expensive chemicals by many schools and at the same time it would
allow students to obtain a significant quantity of the deoxyribonucleic
acid for further investigation. |
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