Natalie Raso - Weapons of Targeted Destruction: Using Viruses to Kill Cancer
     Major Results
Project Information

Abstract

Project Summary

Background

Purpose

Scientific Thought

Hypotheses

Apparatus and Materials

Genetically Engineered KM110red Herpesvirus

Methodology

Procedure for Cell-Line Splitting

Procedure for KM110r Infection

Procedure for Immunofluorescent
Microscopy Imaging

Statistical Analyses

Proliferation Assay Analyzed Data

Major Results

Graphed Results

Discussion of Statistics

Controls and Variables

Conclusions

Discussion

Discussion of KM110r Efficacy

Successes and Failures

Sources of Error and Data

Limitations

Future Research

Applications

Glossary

Bibliography

Acknowledgements 		 
  1. In the 34°C temperature trials, 100% U2OS+ cells were completely dead by time period 4.
  2. In the 34°C temperature trials, the hFOB- and hFOB+ grew at equal rates during the entire experimentation period.
  3. In the 34°C temperature trials, the t-probability of the hFOB- and hFOB+ was 0.59 or 59%.
  4. The MIX+ declined steadily until time period 3 where it began to follow the same growth pattern as the hFOB.
  5. In the 34°C temperature trials, U2OS- and MIX- steadily increased during the experimentation period.
  6. In the 34°C temperature trials no hFOB cells were identified in the RFP channel, therefore none were infected.
  7. In the first 37°C temperature trial, 100% U2OS+ cells were completely dead by time period 3.
  8. In the second 37°C temperature trial, 100% U2OS+ cells were completely dead by time period 2.
  9. In the 37°C temperature trials, the hFOB- and hFOB+ grew at equal rates during the entire experimentation period.
  10. In the 37°C temperature trials, the t-probability of the hFOB- and hFOB+ was 0.989 or 98.9%.
  11. In the 37°C temperature trials the MIX+ grew at a fairly even rate during experimentation, showing no considerable increases or decreases.
  12. In the 37°C temperature trials, U2OS- and MIX- steadily increased during the experimentation period.
  13. In the 37°C temperature trials no hFOB cells were identified in the RFP channel, therefore none were infected.
  14. In the 39°C temperature trials, only 26% cell death occurred by time period 4.
  15. In the 39°C temperature trials, the hFOB- and hFOB+ grew at equal rates during the entire experimentation period.
  16. In the 39°C temperature trials, the t-probability of the hFOB- and hFOB+ was 0.949 or 94.9%
  17. In the 39°C temperature trials the MIX+ grew at a fairly even rate during experimentation, showing no considerable increases or decreases.
  18. In the 39°C temperature trials, U2OS- and MIX- steadily increased during the experimentation period.
  19. In the 39°C temperature trials no hFOB cells were identified in the RFP channel, therefore none were infected.
  20. In the 39°C temperature trials bone-forming nodules began to appear in the hFOB cells.
  21. There were significant differences among the U2OS+ growth rates at each temperature.
  22. The F-ratio for U2OS growth rates at all temperatures, as calculated by the ANOVA, is 15.72.
  23. The significance value for U2OS growth rates at all temperatures, as calculated by the ANOVA, is 0.001 or 0.1%.


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