| Natalie Raso - Weapons of Targeted Destruction: Using Viruses to Kill Cancer | Abstract |
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Project
Information Abstract Project Summary Background Purpose Scientific Thought Hypotheses Apparatus and Materials Genetically Engineered KM110red Herpesvirus Methodology Procedure for Cell-Line Splitting Procedure for KM110r Infection Procedure for Immunofluorescent Microscopy Imaging Statistical Analyses Proliferation Assay Analyzed Data Major Results Graphed Results Discussion of Statistics Controls and Variables Conclusions Discussion Discussion of KM110r Efficacy Successes and Failures Sources of Error and Data Limitations Future Research Applications Glossary Bibliography Acknowledgements |
Safety Equipment ·
Level II
Biosafety Cabinet ·
Laboratory Coat ·
Rubber
Gloves ·
Goggles Splitting for Cell-Line Maintenance (Carried
out in Level II Biohazard Cell Hood) ·
Falcon
T75 filtered containers ·
Phosphate-buffered saline (PBS) ·
Trypsin ·
Dulbecco’s Minimal Essential Medium (DMEM) +
10% Fetal Bovine Serum (FBS) ·
T75
filtered containers ·
Aspirating pipettes connected to draining
system ·
1mL, 5mL,
10mL and 25mL liquid dispenser ·
70%
Ethanol ·
Leica
Light Microscope ·
Incubator Cell Line Splitting to 6-well dish (Carried out
in Level II Biohazard Cell Hood) ·
Falcon
6-well dish ·
Phosphate-buffered saline (PBS) ·
Trypsin ·
DMEM +
10% FBS ·
T75
filtered containers ·
Aspirating pipettes connected to draining
system ·
1mL, 5mL,
10mL and 25mL liquid dispenser ·
70%
Ethanol ·
Leica
Light Microscope ·
Incubator ·
Gilson
Pipetman® (10µl, 200µ, 100µl) and tips ·
Calculator KM110r Infection of Cells in 6-well
Dishes ·
Serum-Free (SF) DMEM ·
Aspirating pipettes connected to draining
system ·
Incubator ·
Gilson
Pipetman® (10µl, 200µ, 100µl) and tips ·
Calculator ·
70%
Ethanol ·
DMEM +10%
FBS Immunofluorescent Microscopic
Imaging ·
Leica® DM IRB
High-Powered Immunofluorescent Microscope System ·
Openlab® Microscope
Software Proliferation Assay ·
Manually
operated Talley cell counter ·
Microsoft
Excel®
Software |