24 hours after the cells were infected, they were immunofluorescently imaged under the Leica DM IRB High-Powered Immunofluorescent Microscope System four times in a five day period. Each session entailed imaging each well in 3 separate fields, and imaging each field in bright field light and GFP and RFP where necessary. This extensive imaging procedure was carried out 24 times over the course of experimentation, as each temperature trial (34, 37°C, and 39°C) was replicated. The steps for performing this procedure are outlined below.

1. Microscope, bulb, and MAC OSX computer system are powered on.
2. The 6-well dish is placed on the microscope stage.
3. The fields of view are navigated using a joystick according to a live feed from the microscope to the computer screen.
4. Random areas are selected from each field, captured, and named according to cell line, status of infection, fluorescent channel, and number of image.
5. Once all images have been taken, they are counted visually using a manually operated Talley cell counter.
6. After each image has been counted, they are entered into a Microsoft Excel Spreadsheet and subsequently graphed. Trends are identified and statistical analyses are carried out.