Natalie Raso - Weapons of Targeted Destruction: Using Viruses to Kill Cancer
    Applications

Project Information

Abstract

Project Summary

Background

Purpose

Scientific Thought

Hypotheses

Apparatus and Materials

Genetically Engineered KM110red Herpesvirus


Methodology

Procedure for Cell-Line Splitting

Procedure for KM110r Infection

Procedure for Immunofluorescent
Microscopy Imaging


Statistical Analyses

Proliferation Assay Analyzed Data

Major Results

Graphed Results

Discussion of Statistics

Controls and Variables

Conclusions

Discussion

Discussion of KM110r Efficacy

Successes and Failures

Sources of Error and Data

Limitations


Future Research

Applications

Glossary

Bibliography

Acknowledgements
 

This research will open new possibilities in the therapy of cancer. The usefulness of the discoveries found in this experiment includes:

 

·        KM110r can be a powerful tool in combating cancer. Because of its specificity, it may prove to be more advantageous over the currently used anticancer drugs which invariably have unwanted toxic side effects. KM110r will only target the osteosarcoma cells without interfering with early precursor cells as they progress to terminally differentiated cells.

·        As a cancer therapy, use of KM110r is optimal when administered at 37°C —or physiological body temperature—since 34°C conditions delay cell destruction, and 39°C conditions do not permit successful termination of the cancerous cells. Therefore, the effectiveness of KM110r as an oncolytic virus therapy could be severely impaired if the body temperature of the cancer patient becomes hyperthermic or hypothermic, i.e. the patient spikes a fever or experiences dangerously low body temperatures, respectively.  A patient with a fever may need an analgesic administered along with the KM110r therapy.

·        KM110r is worth developing into an oncolytic virus therapy

 

In the near future, KM110r can be adopted as a promising therapy against cancer.



Back to Home Page - Natalie Raso