Introduction

Fighting bacteria!

Even though most bacteria in our environment are good, there are also pathogenic bacteria which can cause serious diseases.  This is why we use antibacterial detergents to kill bacteria and reduce the risk of diseases.  In this experiment we will test the efficiency of detergents used in everyday households.

Material

-         ten Petri dishes with agar

-         Q – tips

-         Para film

-         warm place for incubation

-         Household detergents

o       Bleach

o      Dish soap

o      Hand soap   

o      Windex with ammonia               

o      Simple Green                            

o      Tilex               

o      Ear antiseptic

o      Mouth wash                          

Method

-         labeling of dishes

-         wetting of Q-tips with my saliva for 10 sec.  

-         dipping into detergents for 5 seconds

-         inoculation (except control dish) by rubbing Q-tip on agar

-         sealing of dishes with parafilm

-         incubation (3-5 days) at 28ºC

-         observation of colonies and photos

-         storage in fridge

The sealed dishes are observed each day for four days. 

Results

TABLE # 2
Inoculum day day day day   Observations  
  1 2 3 4          
Control-non-inoculated 0 0 0 0          
Control - saliva 1 11 23 25   Presence of fungi along with the colonies
Saliva + bleach 0 0 0 0          
Saliva + dish soap 0 1 3 3   Presence of fungi  
Saliva + hand soap 0 6 18 22   Fungi      
Saliva + Windex 0 11 20 30   Fungi      
Saliva + Simple Green 0 2 2 2   Fungi      
Saliva + Tilex 0 0 0 0          
Saliva + Antiseptique 0 0 0 0          
Saliva + mouth wash 0 0 1 5   White traces    

Table # 2

Like the non-inoculated control, saliva treated with bleach, Tilex, and ear antiseptic do not show any colonies after four days of incubation.

In the other dishes, where saliva was treated with other detergents, colonies are growing.  In some dishes, fungi is also growing.  The handsoap and the Windex have no effect on bacteria and the number of counted colonies is the same as the non-treated saliva control.  Dish soap, Simple Green, and mouth-wash are capable of killing some bacteria because we count less colonies then in the non-treated saliva control.

Conclusion

With this experiment, we have shown that some detergents work and some don’t against bacteria.  In my experiment, I used the detergents either pure or very concentrated compared to normal use.  The Q-tips were only dipped into the detergents for 5 seconds, but normally you would use a detergent for more than 5 seconds.  It is important to note that the Q-tips were just dipped into the detergents; normally, you would rub the surface with the detergent to clean.

Finally, we have shown that many bacteria can resist detergents. However, most of the bacteria that surround us are harmless.