Procedure
Table of contents Abstract Introduction Procedure Results Conclusion Bibliography Acknowledgments Project Information The Germ Wars
Objective: To determine that which type of soap will kill bacteria more effectively.
Hypothesis: I believe antibacterial soap will kill the most bacteria because of the particular chemical ingredient called Triclosan.
Materials:
-Notebook
- D H2O (distilled H20)
- Swabs
- Plates (trypic soy agar plates)
- Permanent marker
- Paper towel
- Soaps (listed below)
- Sunlight (regular soap)
- Jergens (regular soap)
- Dial (antibacterial soap)
- Softsoap (antibacterial soap)
- 2 plastic cylinders (for D H20)
- a dog
- Water (rinse hands)
Variable(s)
Independent: The different varieties of soap.
Dependent: The amount of bacteria that grows of the plates.
Controls:
- The amount of soap I use ( 1/4 teaspoon)
- Temperature of water: hot to wash hands
- Room temperature the same for each plate
- Same amount of time when washing hands
- Always a similar bacteria load
Procedure:
1) Pour D H20 (distilled H20) in a plastic cylinder.
2) Divide each plate into 6 sections using permanent marker.
3) Label each section from 1-6.
4) Rub hands on dog for 10 sec.
5) Swab hands without washing with soap.
6) Repeat this step, but with washing with soap for 10 seconds under warm water using a different type of soap.
7) Swab palm of my hand then swab section number two of plate.
8) Repeat these steps 3 more times, not washing and washing with different soaps and swabbing different sections of the plate.
9) Let the plates sit for 24 hours.
10) Count all the colonies of each section of the after after 24/48/72 hours.
11) Note all results showing the differences between each soap.
12) Repeat this experiment five times.