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Abstract Filamentous fungi (mould) impact humans and are a menace to health. It is one of the leading causes of allergic rhinitis and asthma. Yearly, 20% of Canadians suffer from respiratory allergic symptoms due to moulds. Growing moulds in controlled lab conditions, allow researchers to investigate its impact on humans. High variability exists in moulds from one lab condition to another. Establishing optimum biological experimental condition is often difficult. The media utilized is one of the important ingredients in growing moulds. The present study’s objective is to answer, “Which medium results in effective mould growth?” Synthetic, semi-synthetic and natural mediums are used to grow moulds. A test matrix consisting of over 200 cases were studied using an experimental procedure consists of seven steps. The involved steps are: proper sterilization and maintenance techniques, obtaining culture mould, mould study, media preparation, direct isolation techniques, test conditions and determination. Samples were analyzed using microscopic study by applying an existing mould growth measurement technique. An innovative mould growth technique was identified to overcome the drawback of the existing method. It was concluded that Sabouraud’s Agar, was the most effective medium to grow mould in a laboratory environment. Objective Find a medium which mould growth can grow effectively. Surf at "Background" under "Q & A" for detail understanding of objective. Introduction What you need to know about moulds? Moulds are Where can you find moulds? Mould can be Project Information Prashanthi Baskaran
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