o Cell culture flasks (80 & 28mL)
o Lymphoblastoid cell lines (LCL’s) – GM2184 and GM2669
o 37°C incubator
o diluvials
o pipettes (10, 25mL)
o pipette boy
o pipettors (10, 20, 100, 200mL)
o pipette tips
o Coulter Counter
o blue detergent
o flushing solution
o isoton II
o RPMI 1640 medium
o FBS (Fetal Bovine Serum)
o P/S (Penicillin and Streptomycin)
o L-Glu (L-Glutamine)
o hot water bath
o ethanol
o spray bottle
o tubes (50mL)
o colcemid
o centrifuge tubes
o centrifuge machine
o cold horse serum solution (1:5 horse serum: milli-Q water)
o 5% acetic acid
o fix (3:1 methanol: acetic acid)
o freezer
o glass slides
o kimwipe
o sponge for hot water bath
o UV microscope
o microfuge tubes (500mL)
o NE Buffer
o water
o dNTPs
o primer
o polymerase
o template probes
o Alexa 594 fluor
o TMR fluor
o DEAC fluor
o FITC fluor
o 633 fluor
o mineral oil
o PTC block
o agarose gel containing EtBr (ethidium bromide)
o gel electrophoresis plastic container
o positive and negative electrodes
o voltage source
o 1x TAE solution
o sucrose solution
o ladder solution
o small plastic box
o UV light source
o warming tray
o heating block
o 22mmx30mm plastic cover slips
o 24mmx50mm cover slips
o 60% hybridization buffer solution
o 2X SSC (Standard Sodium Citrate)
o glass Coplin jar
o ethanol series (70%, 80%, 95% ethanol)
o air dry
o rubber cement
o hybridization chamber
o 0.4% SSC and 0.3% NP-40
o thermometer
o 2X SSC and 0.1% NP-40
o DAPI/Antifade solution
o nail polish