Results
1, Growth of B. japonicum 532C and its natural derivatives (designated 532C d1 and 532C d2) in commercial medium.
Viable cell enumeration and optical density indicated that there is no significant difference between 532C parent strain and its natural derivatives. That means no visible change happens to the growth feature in 532C d1 and 532C d2 as compared with the parent strain.
|
|
OD600* |
Viable cell No. Per ml* |
|
532C parent strain |
1.277 |
7.23 x109 |
|
532C d1 |
1.308 |
8.60 x109 |
|
532C d2 |
1.273 |
8.25 x109 |
* Note: The Optic Density is an average of 2 replicas to reduce the chances for serious errors.
2. Survival rates on soybean seed
|
*Viable rhizobia |
Time: 0 |
7 days |
13 days |
20 days |
|
532C |
1.67x106/seed |
2.21x105/seed |
1.01x105/seed |
7.33x104/seed |
|
532C d1 |
1.83x106/seed |
9.33x104/seed |
3.87x104/seed |
1.23x104/seed |
|
532C d2 |
1.71x106/seed |
2.53x105/seed |
1.57x105/seed |
1.13x105/seed |
* Note: average of 2 replica.
Survival percentage on soy seed:
|
|
0 days |
7 days |
13 days |
20 days |
|
532C |
100% |
13.23% |
6.05% |
4.34% |
|
532C d1 |
100% |
5.10% |
2.11% |
0.67% |
|
532C d2 |
100% |
14.80% |
9.18% |
6.61% |