| To
reduce the substrate specificity and terminality of enzymes
involved in degradation of hydrocarbons by directed evolution.
Enzymes with preferential activity towards larger substrates
will be selected in an attempt to push the pathways towards
degradation of high molecular weight substrates.
To attempt
to engineer a multi-enzyme complex. This is a large modular
protein with several catalytic domains (the individual
enzymes) with polyglycine or polyproline linkers, the
optimal length for these linkers would be determined. |
