Directed Evolution of Alkane Degrading Enzymes

References

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• [3] Susanne Brakmann, and Kai Johnsson (2002) Directed Molecular Evolution of Proteins.
• [4] F. Drauz, and H. Waldmann (2002) Enzyme Catalysis in Organic Synthesis.
• [5] Jun Ho Maeng, and Yasuyoshi Sakai, and Yoshiki Tani, and Nobou Kato. (1996) Isolation and Characterization of a Novel Oxygenase that Catalyzes the First Step of n-Alkane Oxidation in Acinetobacter Strain M-1. Journal of Bacteriology. p. 3695-3700.
• [6] Takato Yano, Shinya Oue, and Hiroyuki Kagamiyama (1998) Directed Evolution of an Aspartate Aminotransferase with New Substrate Specificities. Department of Biochemistry, Osaka Medical College.
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• [9] Miriam Sarah Hasson et all. (1998) Evolution of an enzyme active site Natl. Acad. Sci. p. 10396-10401.
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Web Links

PCR, DNA sequencing and in vitro mutagenesis from Strahan & Read
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.611
On-line Primer Generator for site-directed mutagenesis
http://www.med.jhu.edu/medcenter/primer/primer.cgi
Biochemistry Online: An Approach Based on Chemical Logic
http://employees.csbsju.edu/hjakubowski/classes/ch331/bcintro/default.html
Directed Enzyme Evolution from Frances Arnold
http://www.che.caltech.edu/groups/fha/Enzyme/directed.html
Directed Evolution by Recombination
http://ser.bm.udel.edu/~AAnnone/
In Vitro Microbial Genetics outline and sample problems
http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/
LiveWorld Inc: Technologies of Molecular Evolution
http://www.liveworld.com/transcripts/BioOnline/1-25-2001.1-1.html
Maxygen MolecularBreeding(TM)
http://maxygen.com/science-platform.php
The Nobel Prize in Chemistry 1993
http://www.nobel.se/chemistry/laureates/1993/index.html
Weizmann Institute of Science BioGuide to PCR
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html
Biotech Dictionary from the University of Texas
http://biotech.icmb.utexas.edu/search/dict-search.html

Acknowledgements

A year of research could only have been so exciting (and possible) only with the assistance of numerous individuals I would like to mention, beginning with
Dr.Surrette and Dr.Kanti at the Heritage Research Centre, to whom I owe the
best of mentorship and a huge amount of time in teaching that allowed the pursuit of this project with passion. I wish to thank my friend Andrew Stagg and
indeed his entire family for their generosity and support. Thanks also to Aaron
White for help with extracting and standardizing the template DNA. Greetings
again to the professors, students, and acquaintances of Calgary, to their
friendship and understanding!