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- Abstract
- Introduction
- Materials
- Methods
- Detailed Methods
- Results
- Calculations
- Discussion and Relevance
- Conclusion
- References
- Acknowledgements
- Project Info Results

Experiment A

Cells proved to be perfectly cultivated, and were prepared for experiments B, C and D.


Experiment B

Lysed cells were destroyed, and viable cells remained. Under a microscope, using a hand counter, the cells were counted and collected for calculation of hydrogen peroxide required to cause 50% cell death. This, coupled with experiment C, returned the correct required percentage (see results, experiment C). Lysed cells were counted using a 30x30 grid (approx) special tray..


Experiment C

Cells in which the mitochondria was still active turned black, as the Hoechst stain entered the nucleic acid. Those which had a non-reactive mitochondria (dead cells) remained unstained, and transparent. The cells were then placed under a florecent light on the hemocytometer, and cells were counted. A ratio was then setup between original number of cells, and current living cells. This, with equivelent results from experiment B, revealed that to aquire 50% cell death using hydrogen peroxide, 500 microliter at 15 minutes was required.


Experiment D

It was found that wells containing Blueberry extract had the lowest level of cell death among the cells. In blueberry extract with hydrogen peroxide VS without blueberry extract, the latter had greater cell death. Blueberry and hydrogen peroxide versus control and hydrogen peroxide yeilded the same results as previously stated. Also, 0.1% blueberry extract not only provided neoroprotection to cells after hydrogen peroxide exposure but also seemed to cause significant survival in the number of neurites per cell.

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Copyright 2004 Gabriel Rigg, Michael Armson, David Dickinson.
All Rights reserved