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- Home - Abstract - Introduction - Materials - Methods - Detailed Methods - Results - Calculations - Discussion and Relevance - Conclusion - References - Acknowledgements - Project Info |
Shy-Y Neuronal Cells. 10 X 12-well culture plates (Falcon) Collagen substrate (Gibco Life Sciences) Blueberry extract powder (YHC, Tokyo, Japan; Oxford Frozen Foods, NS, Canada) Minimum Essential Media (MEM, Gibco), Fetal bovine serum (FBS, Gibco), Horse serum (HS, Gibco). 1% penicillin/Streptomycin (P/S, Gibco) 7S Nerve Growth Factor (Sigma) L-Glutamate (Sigma) Hanks Balanced Salt Solution (HBSS, Gibco) Zapoglobin cell lysing solution (in HBSS) Viability Staining Kit (Becton Dickson Biosciences) Sterile Pasteur pipettes, Pipettors (0.1-1 ml), sterile pipette tips, eppendorf tubes Incubator Nikon Eclipse Inverted Microscope equiped for epifluorescent Hemocytometer chamber and coverslips Bench microcentrifuge Roche MTT Cell Proliferative Kit I |
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Copyright 2004 Gabriel Rigg, Michael
Armson, David Dickinson. |
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