Materials

MCF-7 cells  (human breast cancer epithelial cell line, from ATCC).

35 mm culture plates (Falcon)

Dulbecco’s Minimum Essential Media (DMEM, Gibco),

Fetal bovine serum (FBS, Gibco)

L-Glutamine (Sigma)

Hanks Balanced Salt Solution (HBSS; Gibco).

Viability Staining Kit (Becton Dickson Biosciences).

Propidium iodide (Sigma)

Sterile Pasteur pipettes, Pipettors (0.1-1 ml), sterile pipette tips, eppendorf tubes

Incubator

Nikon Eclipse Inverted Microscope equipped for epifluorescent

Nikon Elcipse microscope equipped for DIC 

Bench microcentrifuge

Mibefradil (donated from Hoffman La Roche).

Tamoxifen (RBI, Sigma).

Pimozode (Sigma)

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