
Welcome to our website, displaying the research, experimental design, and results of our biotechnology project. This first page gives an introduction to our topic and the source of our ideas. Follow the links below to find the other aspects of our project.
Acknowledgements and Bibliography
Abstract:
This experiment was a constantly evolving process as no work like it had yet been conducted. One of the greatest initial barriers involved with this project was the complete lack of prior research and thus information surrounding specific proteases in rice crops, and thus much of the preliminary work was based on research involving wheat crops. This project first explored the effects of humidity and time on levels of enzyme activity in rice, using an azocasein hydrolysis assay. The levels of protein in each of the different types of rice were then measured using a Bradford reagent. This data was used to determine the necessary sample quantities to be used in zymography, to compare the size of the protein molecules with those of known markers. Finally, the effect of different known and unknown protease inhibitors on rice were tested using the same azocasein assay in hopes of finding some food-grade possibility for industrial use in developing countries.
Introduction:
The United
Nations estimates that over 840 million people in the world suffer from
malnourishment or dietary insufficiency. It is estimated that at least 799
million of these people live in the developing world. With the costs of food
preservation, transportation and preparation being high, especially for
countries with hundreds of millions of people in need, these statistics are
comprehensible. Food preservation is one of the most costly ventures, as the
time required to harvest crops and then ship them to the countries in need is
longer than the time for which it remains fresh and edible. It seems, then, that
a large amount of food spoilage and wastage could be eliminated if food was able
to be kept longer, be it in silos, transport trucks, or markets. With this in
mind, the idea for the following project was developed – the idea to use a
food-grade inhibitor when attempting to prevent spoilage. The idea to use rice
as the experimental subject came about as a result of further research into the
major crops and exports of the world. These crops included maize, rice, oil
palm, and more. In researching past experiments with these plants, few results
were found and it seems that little work has been done. Much lab work has been
conducted, however, using wheat, a common North American crop. Rice was then
chosen because it is a main source of nutrition for the developing world.
Food spoilage is any change which renders a product unacceptable for
human consumption – any process which makes food inedible. Food spoilage
encompasses physical damage, growth of microorganisms, slime formation, or
insect damage. It has been estimated that as much as one quarter of the
world’s food supply is lost to spoilage, namely microbial activity. Therefore,
the enzymatic activity which takes place in sources of nourishment creates an
annual financial and economic strain on many of the world’s nations.
A main problem with the current work towards rectifying this problem of
food spoilage is that the topic is generally inadequately understood. Because of
this fact, it has been difficult to work towards preventing microbial action in
major crops and food stores.
Foods that are highly nutritious and have high moisture content spoil
most rapidly; these foods include meat, poultry, fish, shellfish, milk and some
dairy products. For the most part, food purchased and consumed in developed
countries of the world are properly stored and prepared; the money is readily
available to ensure safe standards of nutritional supplies. In less-developed
countries, however, there is not an adequate supply of money for this high
standard of food preparation to occur. While these rapidly-spoiling foods are
not as commonly consumed in the developing world, their main staples including
rice and maize will also spoil if left unpreserved.
A
grain of rice is the seed of the rice plant, a type of grass. The rice plant has
narrow, tapered leaves and can grow to be anywhere from 60 to 180 centimeters
tall. Several flower stalks grow from each rice plant. Once the grains have
fully developed, the flowers droop with their weight and farmers begin to
harvest. Rice plants require very tropical conditions in order to grow and
survive. Many varieties are grown in flooded wetlands, such as many of those
produced in China.
The rice industry is one that many countries depend upon to feed their
own populations. It is considered to be a significant agricultural crop in over
50 countries worldwide. In fact, rice provides 35-59 percent of the total food
energy consumed by over 2.7 billion people (almost half the world’s
population). In an ever-growing international market such as this, it is crucial
that all of the products produced are of the highest quality. Researchers have
been working for many years to genetically or physically modify rice grains to
ensure that they are both stronger to withstand harvest methods, and also more
nutritionally valuable to those consuming them. However, little progress has
been made in the area of prevention or protection from spoilage.