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Results:
When it was discovered that the pH of the juices were very acidic, it was suspected that their acidity could kill cells (refer to Fig. 1). As was predicted, higher concentrations of the juice, especially cranberry juice, killed a larger amount of cells (refer to Fig. 2). Once the juices were neutralized and used in smaller concentration, they did not kill cells. TNF was then added to the cells along with various concentrations of cranberry and cherry juice (refer to Fig. 3 & Fig. 4). The cranberry juice prevented IL-8 production in unstimulated cells (Fig. 5) while the cherry juice did not prevent the IL-8 production in HT29 cells (Fig. 6). The high levels of IL-8 produced by the stimulated cells could not be read by ELSA (Fig. 5 & Fig. 6). Another ELISA Assay was done with a 1/100 dilution. The results showed that the higher concentrations of cranberry juice inhibit the production of IL-8 (Fig. 7), however, the cherry juice does not inhibit the production of IL-8 (Fig. 8).
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