Myeloid Cancer Blocking by Induction of
Differentiation by Echinacea

 

by Ewa Rajda,
Sacred Heart Shool, Halifax, Nova Scotia

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MATERIALS:

  • J774 tumor cells (from ATCC)
  • Culture Media (RPMI 1640, 10% FBS + 20 mM Hepes + 50µM 2-ME + 1% L-glutamine + 1% Penicillin/Streptomycin)
  • 50 ml tissue culture flaks
  • beaker
  • 15 ml centrifuge tubes
  • cell scraper
  • pipette tips (200 and 1000 µl)
  • pipetter
  • pipettes (5 and 10 ml)
  • pipette aid
  • 96 well immuno plates
  • 96 well tissue culture plates
  • cellulose filter
  • Trypsin (0.05%/0.53µM EDTA)
  • Trypan Blue
  • 3H-Thymidine
  • Interferon Gamma (IFN-y)
  • Lipopolysaccharide (LPS)
  • Echinacea Polysaccharide preparation
  • Liquid Scintillation Beta
  • Centrifuge
  • safety cabinet
  • microscope
  • hemocytometer
  • radioactive container
  • vortex
  • warm water bath
  • incubator
  • Sintillation Beta Counter
  • Semi Automatic Cell Harvester
  • Fluorescent Activated Cell Sorter (FACS)

ELISA Materials:

  • Wash Buffer for ELISA: Phosphate Buffer Saline/ Tween 20 Solution (PBS/Tween Solution)
  • Assay Diluent: 10% Fetal Bovine Serum in Phosphate Buffer Saline (10% FBS in PBS)
  • Working Detector: Biotinylated Antibody + Horseradish peroxidase § Capture Antibody for anti-mouse IL-6
  • Capture Antibody for anti-mouse TNF-a
  • Substrate Solution
  • Phosphoric Acid